The present study investigated the effect of two different hormones (17α-methyltestosterone and 17β-estradiol) on the sex reversal of African catfish (Clarias gariepinus) juveniles. For each hormone type, a total of three treatments and a control were used having different dosages of 100 mg kg-1 (T1), 200 mg kg-1 (T2), 300 mg kg-1 (T3), and 0 mg kg-1 (T0), respectively. The study also gathered the percentage of survival for each hormone. Each hormone was mixed with feed pellet using the alcohol evaporation method. The result of the sex reversal treatment for 17α-methyltestosterone showed that the male population increased as the dosage of hormone increases. Additionally, the sex reversal treatment using 17β-estradiol showed that female populations increased as the dosage of hormones increases. Treatments 1, 2, and 3 of 17α-methyltestosterone showed a significantly higher percentage of males compared to the control having 70.56%, 90.24%, and 100% males respectively. On the other hand, treatments 1, 2, and 3 of 17β-estradiol showed a significantly higher percentage of females compared to the control gathering 76.11%, 92.02%, and 98.89% females respectively. Meanwhile, the highest percentage of survival rate in 17α-methyltestosterone was observed in the control (55±7.0%) which showed a significant difference with treatment 3 (40.33±2.52%) (p < 0.05) who gained the lowest survival rate. Treatments 1 and 2 showed no significant difference among other treatments. On the other hand, treatment 3 of 17β-estradiol with 68.33±2.08% gained the highest survival with a significant difference between treatment 1 (56±3.61%) and the control (55±7.0%) but no significant difference with treatment 2 (58.67±3.79%). The present study proved that the use of 17α-methyltestosterone and 17β-estradiol can successfully masculinize and respectively feminize African catfish.
