The use of contaminated brine shrimp, Artemia franciscana (Kellog, 1906), as live feed leads to vibriosis outbreaks and threats to global aquaculture productivity. This study highlights a potential cost-effective and environmentally friendly approach to suppress Vibrio spp. load in Artemia live feed. Axenic A. franciscana nauplii were reared with Vibrio campbellii and Vibrio parahaemolyticus under 30-150 PSU of salinity. A salinity of 80 PSU can eliminate more than 90% of both strains, while maintaining over 90% survival of A. franciscana nauplii. Another experiment demonstrated that the Vibrio spp. load in non-axenic cyst-hatching tanks at 30 PSU was high but reduced with increasing salinities. The addition of 1 g L-1 Pandanus tectorius leaf extract in the high salinity rearing water significantly reduced cyst-derived bacterial load. Findings showed that 80-110 PSU of salinity is effective in suppressing almost all the Vibrio spp. without adverse effects on A. franciscana growth, removing the need for cyst disinfection or nauplii tank transfer.
